Cloning, expression and characterization of a chitin-binding domain

Cloning, Expression and Purification of Clostridium botulinum Neurotoxin Type E Binding Domain

Cloning of Binding Domain of Clostridium botlllinum Toxin Type A in Escherichia coli

Expression and characterization of the chitin-binding domain of chitinase A1 from Bacillus circulans WL-12.

Chitinase A1 from Bacillus circulans WL-12 comprises an N-terminal catalytic domain, two fibronectin type III-like domains, and a C-terminal chitin-binding domain (ChBD). In order to study the biochemical properties and structure of the ChBD, ChBD(ChiA1) was produced in Escherichia coli using a pET expression system and purified by chitin affinity column chromatography. Purified ChBD(ChiA1) spe...

Gene cloning and expression of soluble thrombopoietin functional domain by harnessing Rosetta-gami expression system

Background: Thrombopoietin (TPO) is an important cytokine that has a critical role in regulating hematopoietic stem cells (HSCs) proliferation and megakaryocyte differentiation. Because of scares amount of this protein in human plasma, in many biotechnological centers around the world, recombinant production of this protein has been carried out. This study was aiming to gene cloning and express...

Cloning of conserved regions of nontypeable Haemophilus influenzae hmw1 core binding domain

Colonization of nontypeable Haemophilus influenzae (NTHi) in nasopharynx causes respiratory tract disease. In 80% of clinical isolates, HMW proteins are the major adhesions and induce protective antibodies in the hosts. Therefore, it can be used as a vaccine candidate. The aim of this study is designing and cloning of the conserved regions of NTHi hmw1 core binding domain.In this study, the sta...